The Johnny Appleseed Educational Center & Museum on Urbana University's campus in Urbana, Ohio holds the largest collection of memorabilia and written information about the life of John "Appleseed" Chapman known to exist in the world. A computer database is also available for research purposes for retrieval and identification of information. A National Registry of Johnny Appleseed's relatives has also been established for those interested in genealogical heritage.
Special items on display at the museum include a cider press (circa 1850) that was used by John James to process apples from trees planted by Johnny Appleseed in Champaign County, commemorative plates from festivals held in his honor, wood and bark from original trees planted by Johnny Appleseed in Ohio and Indiana, photos of monuments and markers dedicated to Johnny Appleseed and a portion of the many publications about Johnny Appleseed's life and legend that have been collected. In 1999, seedlings from the last known-surviving apple tree planted by Johnny Appleseed were transplanted in the courtyard around the museum. The trees are testimony to Johnny Appleseed's everlasting contributions to agricultural stewardship.